After the fall of Tiwanaku Empire, the many Aymara Lake Titicaca were conquered by the Inca Empire. Prior to the Spanish conquest, the Andean province of Qullasuyu was a part of the Inca empire, while the northern and eastern lowlands were inhabited by independent nomadic tribes. Spanish conquistadors, arriving from Cuzco and Asunción took control of the region in the 16th century. During most of the Spanish colonial rule, Bolivia was known as Upper Peru and administered by the Royal Audiencia of Charcas. After the 1st call for independence in 1809, 16 years of war followed before the establishment of the Bolivian Republic, named for the Liberator Simón Bolívar, on 6 August 1825. Since then Bolivia has endured regular periods of political and economic instability, including the loss of various provinces to its neighbors, such as Acre, parts of the Gran Chaco and its Pacific coast, making it a land-locked country.